Patents Details





Patent No/Application No: 245749 Title : Process enabling simultaneous detection of two transgenes namely 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS or CP4EPSPS) gene and cauliflower
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Status

Granted

Inventor's Name

Randhawa GJ, Firke PK and Karihaloo JL

Commercialization

Available on Non exclusive basis

Abstract

The present invention comprises a process enabling simultaneous detection of two transgenes using a Multiplex Polymerase Chain Reaction (MPCR), particularly the detection of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS or CP4EPSPS) gene (which imparts resistance against herbicide glyphosate) and cauliflower mosaic virus (CaMV) 35S promoter gene in transgenic maize for herbicide tolerance. The invention can be successfully employed in rapid and simultaneous detection of these two transgenes in transgenic maize with high reproducibility and sensitivity.

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Website

http://www.ipindia.nic.in/

Patent No/Application No: 254341 Title : Process enabling simultaneous detection of two transgenes namely human serum albumin (HAS) and bar genes using a multiplex polymerase chain reaction
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Status

Granted

Inventor's Name

Randhawa GJ, Firke PK and Karihaloo JL

Commercialization

Available on Non exclusive basis

Abstract

Process enabling simultaneous detection of two transgenes namely human serum albumin (HAS) and bar genes using a multiplex polymerase chain reaction (MPCR) utilizing a combination of novel primers and polymerase chain reaction (PCR) protocol in transgenic wheat". The present invention comprises a process enabling simultaneous detection of two transgenes i.e. human serum albumin (HAS) gene responsible for human blood serum protein production as biopharmaceutical product and bar gene imparting resistance against herbicide phosphimothrecin in transgenic wheat using a Multiplex Polymerase Chain Reaction (MPCR). The process comprises (i) Designing of the primer sequence for human serum albumin (HAS) and bar gene (ii) Preparation of polymerase chain reaction mixture (iii) Amplification of target sequence with three temperatures multiplex polymerase chain reaction (iv) Separation of amplified fragments on 3% agarose gel and staining with ethidium bromide. The process developed in the present invention is much more economical, rapid and precise than the traditional polymerase chain reaction. Bar gene is used as the most common selectable marker sequence to transform several commercialized transgenic crops. Multiplex polymerase chain reaction process can confirm that a plant is genetically modified as bar gene along with human serum albumin (HAS) gene can be detected in single experiment, thus the process is much more reliable also for detection/screening of transgenic crops because the transgenic wheat for human serum protein production and herbicide tolerance targeted in this invention has been imported in India will be commercially grown in the country and other countries as well. It has become all the more important to develop reliable, efficient and precise protocols for detection of the transgenes incorporated in wheat i.e. human serum albumin (HAS) gene and bar gene to meet the regulatory requirement and to solve the legal disputes in future if they arise.

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Website

http://www.ipindia.nic.in/

Patent No/Application No: 258165 Title : Diagnostic kit based on polymerase chain reaction for detection of cry1Ac gene
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Status

Granted

Inventor's Name

Randhawa G J, and Firke PK

Commercialization

Available on Non exclusive basis

Abstract

The present invention comparising a process enabling detection of transgene i.e. cryAc gene responsible for production of cry1Ac protein for insert resistance in Bt cotton bollgard-1 using Polymerase Chain Reaction. the process comparises designing of the primer sequence for cry1Ac gene, Preparation of polymerase chain reation mixture, amplification of target sequence using polymerase chain reaction with three temperatures regime and conversion of the technolgy into form of a kit.

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Website

http://www.ipindia.nic.in/

Patent No/Application No: 4279/DEL/2015 Title : A SOXHLET EXTRACTOR
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Status

Pending

Inventor's Name

Dr. R.K. Bhardwaj,Dr. C. Raghav

Commercialization

Available on Non exclusive basis

Abstract

The present invention relates to an improved soxhlet extractor. More particularly the present invention relates to a compact extractor having a unique feature of conserving heat and facilitating warm extraction, solvent entering directly in thimble thereby enabling more number of extraction cycles in the same time.

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Website

http://www.ipindia.nic.in/

Patent No/Application No: 4278/DEL/2015 Title : A REUSABLE MULTIPURPOSE EXTRACTION THIMBLE APPARATUS
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Status

Pending

Inventor's Name

Dr. R.K. Bhardwaj,Dr. C. Raghav

Commercialization

Available on Non exclusive basis

Abstract

The present invention relates to an extraction apparatus. More particularly the present invention relates to an improved extraction thimble and method of extraction. Furthermore, particularly the present invention relates to a unique design for extraction thimble.

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Website

http://www.ipindia.nic.in/

Patent No/Application No: 4277/DEL/2015 Title : A CENTRALLY FUNNELLED VALVED OR NON-VALVED CONDENSER
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Status

Pending

Inventor's Name

Dr. R.K. Bhardwaj,Dr. C. Raghav

Commercialization

Available on Non exclusive basis

Abstract

The present invention relates to a centrally funnelled condenser comprising a cylindrical tube having a hollow interior defined by an outer case; the said cylindrical tube having a lower end and an upper end; the upper end having a hollow venting tube; the lower end having a ground male joint; a base comprising a plurality of elevated holes and a funnel at the centre; a cooling tube or coil being disposed in the interior of the said cylindrical tube.

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Website

http://www.ipindia.nic.in/